Glucose-induced Metabolic Oscillations Parallel Those of Ca2+ and Insulin Release in Clonal Insulin-secreting Cells
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چکیده
منابع مشابه
Ca(2+)-induced Ca2+ release in insulin-secreting cells.
The sulphydryl reagent thimerosal (50 microM) released Ca2+ from a non-mitochondrial intracellular Ca2+ pool in a dose-dependent manner in permeabilized insulin-secreting RINm5F cells. This release was reversed after addition of the reducing agent dithiothreitol. Ca2+ was released from an Ins(1,4,5)P3-insensitive pool, since release was observed even after depletion of the Ins(1,4,5)P3-sensitiv...
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Phospholipase C (PLC) is a ubiquitous enzyme involved in the regulation of a variety of cellular processes. Its dependence on Ca2+ is well recognized, but it is not known how PLC activity is affected by physiological variations of the cytoplasmic Ca2+ concentration ([Ca2+](i)). Here, we applied evanescent wave microscopy to monitor PLC activity in parallel with [Ca2+](i) in individual insulin-s...
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In the mouse beta-cell, glucose generates large amplitude oscillations of the cytosolic-free Ca(2+) concentration ([Ca(2+)](i)) that are synchronous to insulin release oscillations. To examine the role played by [ Ca(2+)](i) oscillations in the process of insulin release, we examined the effect of plasma membrane Ca(2+)-ATPase (PMCA) overexpression on glucose-induced Ca(2+) oscillations and ins...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2001
ISSN: 0021-9258
DOI: 10.1074/jbc.m105056200